Clone ID: EP2259IHCY
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2221-1
Size: 100
Price: $ 285
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| A. Western blot analysis on A431 cell lysates using anti-Phospho-EGFR (pS1070/1071) RabMAb (cat. #2221-1), 1:1,000,000 dilution. Cells were either (A) untreated (B) treated with EGF |
B. Immunofluorescence staining of A431 cells using anti-EGFR RabMAb (cat. # 2221-1). |
Recommended Applications: WB, ICC, IP
Species Reactivity: Human
Mol. Weight: 180 kDa
UniProt ID: P00533
Alternative names: Receptor tyrosine-protein kinase ErbB-1
Description: Epidermal growth factor receptor (EGFR) is a 170 kDa transmembrane glycoprotein receptor tyrosine kinase that, when activated by epidermal growth factor (EGF), affects cell growth and differentiation in normal and cancer cells (1). Binding of EGF or TGF alpha to EGFR activates tyrosine kinase activity of the receptor (2). The carboxy terminal tyrosine residues on EGFR, Tyr 1068, Tyr 1148, and Tyr 1173, are the major sites of autophosphorylation, which occurs as a result of EGF binding (3). EGFR overexpression is exhibited various cancer, such as glioma, colorectal carcinoma, breast carcinoma, and head and neck carcinoma (4). Phosphorylation at Ser 1070 has been implicated in altered receptor desensitization and receptor internalization modification of EGFR (5). Specificity: A synthetic phospho-peptide corresponding to residues surrounding Serine 1070 and 1071 of human EGF receptor was used as immunogen.
Recommended Dilutions:
WB: 1:500,000
ICC: 1:100 - 250
IP: 1:30
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
Hunter T. et al. Nature 311: 414 |
| 2. |
Reynolds FH, et al. Nature 292: 259 |
| 3. |
Helin K, et al. Oncogene 6: 825 |
| 4. |
Christensen ME, et al. Danish Med Bull 45(2):121-134, 1998. |
| 5. |
Countaway JL, et al. JBC 267:1129-1140, 1992 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
