Clone ID: EP2107Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2214-1
Size: 100
Price: $ 285
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A. Western blot analysis on NIH 3T3 cell lysates using anti-Phospho-Akt1 (pT308) RabMAb (cat. #2214-1), 1:10,000 dilution. Cells were either (A) untreated (B) treated with PDGF |
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Recommended Applications: WB
Species Reactivity: Human, Mouse
Mol. Weight: 60 kDa
UniProt ID: P31749
Alternative names: RAC-PK-alpha, Protein kinase B, PKB, C-AKT
Description: Akt1/PKBa is a Ser/Thr protein kinase, whose kinase activity plays a key role in various cellular functions, including apoptosis, glycogen synthesis, and cell growth. Akt1/PKBa is activated by various growth and survival factors (1). Phosphorylation on Thr308, Ser473 and Tyr474 is required for full activity. Akt1/PKBa inhibits apoptosis by phosphorylating and inactivating several targets, including caspase-9 (2). Akt1/PKBa regulates glycogen synthesis by phosphorylating and inactivating glycogen synthase kinase-3a and b (3, 4). Three mammalian Akt1/PKBa isoforms have been identified. Specificity: A phospho specific peptide corresponding to residues surrounding Threonine 308 of human Akt1 was used as immunogen. The antibody detects Akt1 phosphorylated at Threonine 308.
Recommended Dilutions:
WB: 1:5,000
Protocols for using Epitomics Rabbit Monoclonal Antibodies
References:
| 1. |
Burgering, B.M. and P.J. Coffer.Nature 376: 599 |
| 2. |
Cardone, M.H., et al. Science 282: 1318 |
| 3. |
Hajduch, E., et al. FEBS Lett. 492: 199 |
| 4. |
Cross, D.A., et al. Nature 378: 785-789, 1995. |
Orders:
877-772-2622 (US and Canada)
650-443-2276 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
