Clone ID: EP356(2)AY
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2204-1
Size: 100ul
Price: $ 250
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| A. Western blot analysis on HepG2 cell lysate using anti-p53 Acetyl (K373) RabMAb (cat. # 2204-1), dilution 1:100,000. Cells were either (A) untreated or (B) treated with etoposide + trichostatin A. |
B. Immunohistochemical analysis of paraffin-embedded human breast ductal carcinoma using anti-p53 RabMAb (cat. #2204-1). |
Recommended Applications: WB, IHC, ICC
Species Reactivity: Human
Mol. Weight: 53 kDa
UniProt ID: p04637
Alternative names: Tumor suppressor p53, Phosphoprotein p53, Antigen NY-CO-13
Description: p53 acts as both a tumor-suppressor and transcription factor that, upon activation by DNA damage and other cellular stress signals, leads to the transcription of genes triggering cell-cycle arrest, apoptosis, and DNA repair (1,2). The gene for the nuclear phosphoprotein p53 is the most commonly mutated gene yet identified in human cancers (3). p53 is frequently mutated or inactivated in about 60% of cancers. p53 acetylation at K373 is confirmed to be required for recruitment of p300 to the p21 promoter, and the depsipeptide-induced p53 acetylation at K373 is unlikely to be dependent on p53 phosphorylation at Ser15, Ser20, and Ser392 sites. Data suggest that p53 acetylation at K373 plays an important role in depsipeptide-induced p21expression (4,5). Specificity: A synthetic acetylated peptide corresponding to residues surrounding Ly373 of human p53 was used as an immunogen. The antibody only detects p53 acetylated on Lysine 373.
Recommended Dilutions:
WB: 1:50,000
IHC: 1:100
ICC: 1:100
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
Levine, A.J. 88: 323 |
| 2. |
Lakin, N.D et al. Oncogene 18: 7644 |
| 3. |
Vogelstein, B. Nature 348: 681 |
| 4. |
Hoolstein, M., et al. Science 253: 49 |
| 5. |
Harris C.C. Science 262: 1980 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
