Clone ID: EP2136Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2197-1
Size: 100ul
Price: $ 285
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| A. Western blot analysis on NIH3T3 cell lysates using anti-Phospho-PDGF-Receptor β (pY857) RabMAb (cat. #2197-1), 1:1000 dilution. Cells were either (A) untreated (B) treated with PDGF. |
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Recommended Applications: WB
Species Reactivity: Mouse
Mol. Weight: 190 kDa
UniProt ID: P09619
Alternative names: PDGF-R-beta, CD140b antigen
Description: Human platelet-derived growth factor (PDGF) receptor, is a receptor tyrosine kinase whose activity induces various signalling cascades leading to cell growth and proliferation. Phosphorylation of Tyrosine 857 (pY857) in the catalytic loop of the PDGF Receptor beta has been shown to increase kinase activity. Two major isoforms exist, PDGF receptor-α and PDGF receptor-β. PDGF receptor-β can only bind two specific PDGF isoforms, PDGF-β and PDGF-Δ (1,2). Upon ligand binding, the receptor undergoes autophosphorylation, allowing binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules such as PI kinase (3), Grb2, and Src, among others. Specificity: A phospho specific peptide corresponding to residues surrounding Tyrosine 857 of human PDGF-Receptor β was used as an immunogen. This antibody detects PDGF-Receptor β phosphorylated at Tyrosine 857
Recommended Dilutions:
WB: 1:500 - 1000
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
Deuel, T.F., et al. Biofactors 1: 213 |
| 2. |
Deuel, T.F., et al. J Clin Invest. 74: 669 |
| 3. |
Kazlauskas, A, et al. Cell 58: 1121 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
