Clone ID: EP42Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2190-1
Size: 100ul
Price: $ 285
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| A. Western blot analysis on HepG2 cell lysates using anti-p53 Phospho (pS46) RabMAb (cat. #2190-1), 1:2000 dilution. Cells were either (A) untreated (B) treated with etopside. |
B. Immunohistochemical analysis of human ovarian adenocarcinoma using anti-p53 Phospho (pS46) RabMAb (Cat# 2190-1) |
Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 53 kDa
UniProt ID: P04637
Alternative names: Tumor suppressor p53, Phosphoprotein p53, Antigen NY-CO-13
Description: p53 acts as both a tumor-suppressor and transcription factor that, upon activation by DNA damage and other cellular stress signals, leads to the transcription of genes triggering cell-cycle arrest, apoptosis, and DNA repair (1,2). The gene for the nuclear phosphoprotein p53 is the most commonly mutated gene yet identified in human cancers (3). p53 is frequently mutated or inactivated in about 60% of cancers. Due to UV-induced DNA damage, p53 is phosphorylated on eight different serine/threonine locations on the N-terminal. Phospholyation on serine 46 has been implicated as a mediator in apoptosis induced by DNA-damage (4, 5). Specificity: A synthetic phospho-peptide corresponding to residues surrounding serine 46 of human p53 protein.
Recommended Dilutions:
WB: 1:1000 - 2000
IHC: 1:100
ICC: 1;50
Flow Cytometry: 1:40
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
Levine, A.J.; Cell 88: 323 |
| 2. |
Lakin, N.D. S.P. Jackson. Oncogene 18: 7644-7655,1999 |
| 3. |
Vogelstein, B.; Nature 348: 681 |
| 4. |
Hoolstein, M., et al. Science 253: 49 |
| 5. |
Saito, S., et al. J. Biol. Chem. 277:12491 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
