Clone ID: EP233(2)Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2187-1
Size: 100ul
Price: $ 285
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| A. Western blot analysis on MCF-7 cell lysates using anti-Phospho-p27 Kip1 (pS10) RabMAb (cat. #2187-1), 1:10,000 dilution. Cells were either (A) untreated (B) treated with AP. |
B. Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using anti-Phospho-p27Kip1 RabMAb (cat. #2187-1) |
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| C. Immunohistochemical analysis of paraffin-embedded human tonsils using anti-Phospho-p27Kip1 RabMAb. (cat. # 2187-1). |
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Recommended Applications: WB, IHC, IP
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 27 kDa
UniProt ID: P46527
Alternative names: Cyclin-dependent kinase inhibitor p27, p27Kip1
Description: p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1) (2); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3). Specificity: A phospho specific peptide corresponding to residues surrounding Serine 10 of human p27 was used as an immunogen. This antibody detects p27 phosphorylated at Serine 10.
Recommended Dilutions:
WB: 1:10,000
IHC: 1:100
IP: 1:30
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
Polyak K., et al; Cell 78:59-66,1994. |
| 2. |
Carrano,A.C., et al. Nat. Cell Biol., 1, 193-199 |
| 3. |
Rodier,G.,et al. EMBO J., 20, 6672-6682, 2001 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
