Clone ID: EP1998Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2158-1
Size: 100ul
Price: $ 250
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| A. Western blot analysis on (A) Jurkat and (B) HEK293 cell lysates using anti-Presenilin-1 RabMAb (cat. # 2158-1), 1:2000 dilution. |
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Recommended Applications: WB, FC
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 28-35 kDa
UniProt ID: P49768
Alternative names: PS-1, Protein S182
Description: Presenilin (PS) facilitates gamma-secretase cleavage of the beta-amyloid precursor protein and the intramembraneous cleavage of Notch1 (1). The discovery that a deficiency of presenilin 1 (PS1) decreases the production of amyloid beta-protein (Abeta) identified the presenilins as important mediators of the gamma-secretase cleavage of beta-amyloid precursor protein (APP). It has been shown that two conserved transmembrane (TM) aspartates in PS1 are critical for Abeta production, providing evidence that PS either functions as a required diaspartyl cofactor for gamma-secretase or is itself gamma-secretase. Presenilin 2 (PS2) shares substantial sequence and possibly functional homology with PS1. It has been shown that the two TM aspartates in PS2 are also critical for gamma-secretase activity, providing further evidence that PS2 is functionally homologous to PS1 (2). PS are found primarily within intracellular membranes, including the endoplasmic reticulum (ER) and the trans-Golgi network as well as the plasma membrane. PS are also expressed in most cell types throughout development (3). Specificity: A synthetic peptide corresponding to residues NTF subunit of Presenilin-1 protein was used as an immunogen.
Recommended Dilutions:
WB: 1:500 - 2000
Flow Cytometry: 1:10
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
Steiner H, et al. J Biol Chem. 274(40):28669-73, 1999. |
| 2. |
Kimberly WT, et al. J Biol Chem 275(5):3173-8, 2000. |
| 3. |
Kopan et al. Gene Dev. 14(22):2799-2806, 2000. |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
