Clone ID: EP1890Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2152-1
Size: 100ul
Price: $ 285
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| A. Western blot analysis on HEK293 cell lysates using anti-ATM Phospho (pS1981) RabMAb (cat # 2125-1), dilution at 1:10,000. Cells were either (A) untreated or (B) treated with doxorubicin. |
B. Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using anti-ATM Phospho (pS1981) RabMAb (cat# 2152-1). |
Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human
Mol. Weight: 370 kDa
UniProt ID: Q13315
Alternative names: Ataxia telangiectasia mutated, A-T, mutated
Description: ATM is a nuclear phosphoprotein involved in Ataxia-telangiectasia (A-T) an autosomal recessive disorder characterized by cerebellar ataxia, immune deficiencies, increased cancer predisposition, chromosomal instability and radiation sensitivity (1-2). This large protein (370 kDa) is involved in genome stability, cellular responses to DNA damage and cell cycle control. Autophosphorylation at serine 1981 on ATM is required during ATM kinase activation initiated by double strand breaks in genome. After serine 1981 autophosphorylation, ATM phosphorylates downstream proteins (e.g. p53, Chk2) leading to cell cycle arrest and DNA repair (3). More than 100 mutations have been identified so far and are expected to inactivate the ATM protein by truncation or large deletions (4). Defects in ATM contribute to various B cell and T cell Leukemia (5). Specificity: A synthetic peptide corresponding to residues surrounding serine 1981 of human ATM was used as an immunogen.
Recommended Dilutions:
WB: 1:5000 - 10000
IHC: 1:100
ICC: 1: 100 - 250
IP: 1:40
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
Harnden, D.G.; Int. J. Radiat. Biol. 66:S13-S19, 1994. |
| 2. |
Lavin MF, Shiloh,Y. Ann. Rev. Imm. 15:177-202, 1997 |
| 3. |
Goldstine JV, et al. DNA Repair, 5:432-443, 2006. |
| 4. |
Savitsky K et al. Hum. Mol. Gen. 4:2025-2032, 1995. |
| 5. |
Stankovic T.et al. Lancet 353:26-29., 1999. |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
