Clone ID: EP1629Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2147-1
Size: 100ul
Price: $ 285
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| A. Western blot analysis on HeLa cell lysates using anti-Phospho-CK-8 (pS23) RabMAb (cat. #2147-1), 1:50,000 dilution. Cells were either (A) untreated (B) treated with Calyculin |
B. Positive immunohistochemical staining of paraffin-embedded human gastric adenocarcinoma using anti-CK-8 Phospho-RabMAb (cat. #2147-1) |
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| C. Positive immunohistochemical staining of paraffin-embedded human breast adenocarcinoma using anti-CK-8 Phospho- RabMAb (cat. # 2147-1). |
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Recommended Applications: WB, IHC, IP
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 52 kDa
UniProt ID: P05787
Alternative names: Cytokeratin-8, CK-8, Keratin-8, K8
Description: Keratin polypeptides 8 and 18 (K8/18) are intermediate filament phosphoglycoproteins that are expressed preferentially in glandular epithelia. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells (1-3). Together with actin microfilaments and microtubules, keratin filaments make up the cytoskeletons of vertebrate epithelial cells, forming alpha-helical coiled-coil dimers which associate laterally and end-to-end to form 10-nm diameter filaments (4). Specificity: A phospho specific peptide corresponding to residues surrounding serine 23 of human CK-8 was used as an immunogen. This antibody detects CK-8 phosphorylated on serine 23.
Recommended Dilutions:
WB: 1:10,000
IHC: 1:100
ICC: 1:100
Flow Cytometry: 1:20
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
Ku, N.O, et al. J Biol Chem. 270: 11820 |
| 2. |
Kulesh, D.A. et al, Mol Cell Biol. 8: 1540 |
| 3. |
Marceau, N. et al. Biochem. Cell Biol. 73: 619 |
| 4. |
Fuchs, et al. 11: 123 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
