Clone ID: PEG-B-47b
Antibody Type: Rabbit Monoclonal IgG, Biotinylated
Catalog#: 2137-1
Size: 100ug
Price: $ 305
*please call for quote on bulk orders.
Recommended Applications: WB, ELISA, IHC
For more info, vist the Anti-PEG Antibodies Portal
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| A. ELISA assay using 5ug/mL Anti-AFP RabMAb (#1791-1) as the capture antibody and 0.5 ug/mL Anti-PEG-47-Biotin RabMAb (cat. # 2137-1) as the detection antibody. HRP-avidin (1:3000) used for anti-PEG-47 detection. This ELISA assay could detect YCA1017 peptide-PEG antigen at a concentration as low as 0.9 ng/mL |
B. Sandwich ELISA assay using 5ug/mL Anti-GCSF as the capture antibody and 0.5 ug/mL Anti-PEG-47-Biotin RabMAb (cat. # 2137-1) as the detection antibody. HRP-avidin (1:3000) used for anti-PEG-47 detection. This ELISA assay could detect GCSF-PEG antigen at a concentration as low as 0.9 ng/mL |
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| C. Direct ELISA assay using 63 ng/mL of antigen (GCSF-PEG) to coat the plate. HRP-avidin (1:3000) was used for the detection of varying amounts of Anit-PEG-47-Biotin (cat. # 2137-1) |
D. Immunoblot of different antigen-PEG preparations. Western Blot images using Anti-PEG 47-Biotin (cat. # 2137-1) to detect BSA-PEG. Lane 1: 31.25 ng/mL; lane 2: 15.6 ng/mL; lane 3: 7.8 ng/mL |
Description: Polyethylene glycol（PEG）is a family of long chain polymers attached to a glycerine backbone. It is a nonionic, nontoxic, biocompatible, strongly hydrophilic polymer, which has a large exclusion volume in aqueous solution (1). The covalent attachment of PEG is now commonly used to modify a variety of proteins and drugs (2,3). The modification of a biopharmaceutical with polyethylene glycol (PEG) increases its hydrodynamic radius, reduces immunogenicity and proteolytic cleavage. Other benefits include decelerated renal excretion, improved stability towards proteolysis and increased solubility of the biopharmaceutical in aqueous solutions (4). As examples, PEG-adenosine deaminase (Adagen) is used for the treatment of severe combined immunodeficiency syndrome, PEGasparaginase (Oncaspar) is used for the treatment acute lymphoblastic leukemia, PEGinterferon α2a (Pegasys) is used for the treatment Hepatitis C, Branched PEGanti-VEGF aptamer (Pegaptanib, Macugen) is used for the treatment Macular degeneration(age-related) (5). An Anti-PEG antibody can be used to monitor a drugs pharmacokinetics, including distribution, metabolism and excretion. In addition, it can be used for the quality control of pegylated molecules in ELISA, WB and flow cytometery. Specificity: KLH-PEG with terminal methoxy group was used as an immunogen. This antibody recognizes the terminal methoxy group of the PEG molecule. Anti-PEG-47-Biotin is prepared by the biotinylation of the antibody via primary amines.
Recommended Dilutions:
WB: 15.6 ng/mL
Protocols for using Epitomics Rabbit Monoclonal Antibodies
References:
| 1. |
Guiotto, A. et al. (2004) Anchimeric assistance effect on regioselective hydrolysis of branched PEGs: a mechanistic investigation. Bioorg. Med.Chem. 12, 5031 |
| 2. |
Wong, S.S. (1991) Reactive groups of proteins and their modifying agents. In Chemistry of protein conjugation and cross-linking, p. 13, CRC Press |
| 3. |
Caliceti, P. et al.(1993) Active site protection of proteolytic enzymes by poly(ethylene glycol) surface modification. J. Bioact. Comp. Polym. 8,41 |
| 4. |
Frank Leenders, celares GmbH, Berlin, Germany.(2006) PEGylation technology and biopharmaceuticals. Biopharmaceuticals. 6,39-40 |
| 5. |
Francesco M.Veronese, Gianfranco Pasut. (2005) PEGylation, successful approach |
Orders:
877-772-2622 (US and Canada)
650-443-2276 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
