Clone ID: EPR269Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2132-1
Size: 100ul
Price: $ 285
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| A. Western blot analysis on K562 cell lysates using anti-Phospho-Crk/p38 (pY221) RabMAb (cat. #2132-1), 1:2000 dilution. Cells were either (A) untreated (B) treated with AP. |
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Recommended Applications: WB, ICC
Species Reactivity: Human
Mol. Weight: 38 kDa
UniProt ID: P46108
Alternative names: p38, Adapter molecule crk
Description: Crk (p38) are adaptor proteins, composed mostly of SH2 and SH3 domains, which play a key role in cellular signaling by mediating the construction of protein complexes and also by managing cell proliferation, differentiation, and migration (1). They are critical integrators of upstream signals for cell invasion and migration in human cancer cell lines. Crk is believed to be a regulator of invasive responses because increased levels of the protein have been observed in multiple human cancers (2). In vivo studies have demonstrated that decreased levels of Crk remarkably inhibits tumor formation and its invasive growth (3). Crk associates with ERM family proteins (including ezrin, radixin, and moesin), activates RhoA, and promotes cell motility toward hyaluronic acid (4). Specificity: A phospho specific peptide corresponding to regions surrounding Tyrosine 221 was used as an immunogen. This antibody detects proteins phosphorylated at Y221.
Recommended Dilutions:
WB: 1:1000 2000
ICC: 1:50 -100
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
Nishihara H, et al. Cancer Lett (1):55-61, 2002 |
| 2. |
Watanabe T, et al. Mol Cancer Res. (7):499-510, 2006 |
| 3. |
Rodrigues SP, et al. Mol Cancer Res. (4):183-94, 2005 |
| 4. |
Tsuda MJ, et al. Biol Chem. (45):46843-50, 2004 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
