Clone ID: EP1326Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2119-1
Size: 100ul
Price: $ 250
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| A. Western blot analysis on HeLa cell lysate using anti-Cytochrome C RabMAb (catalog. #2119-1), dilution 1:500. |
B. Immunohistochemical analysis of paraffin-embedded human heart muscle using anti-Cytochrome C RabMAb (cat# 2119-1) |
Recommended Applications: WB, IHC, ICC, FC
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 14 kDa
UniProt ID: p99999
Alternative names:
Description: Cytochrome c is located in the mitochondria of all aerobic cells and is involved in the electron transport system that functions in oxidative phosphorylation. It accepts electrons from cytochrome b and transfers them to cytochrome oxidase. In the process, the iron of the heme group, which is identical to that of hemoglobin and myoglobin, shifts from the ferrous to the ferric state (1). Recent progress in studies on apoptosis has revealed that cytochrome c is a pro-apoptotic factor. It is released from its places on the outer surface of the inner mitochondrial membrane at early steps of apoptosis and, combining with some cytosolic proteins, activates conversion of the latent apoptosis-promoting protease pro-caspase-9 to its active form. Cytochrome c release can be initiated by the pro-apoptotic protein Bax. This process is blocked by the anti-apoptotic proteins Bcl-2 and Bcl-xL (2). Also, differences in amino acid composition of cytochrome c have also been used as markers of biochemical evolution. Specificity: A synthetic peptide corresponding to residues near the N-terminus of human Cytochrome C was used as an immunogen.
Recommended Dilutions:
WB: 1:250
IHC: 1:100
ICC: 1:50
IP: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
Wojtczak L, et al. Postepy Biochem 52(2):122-8, 2006. |
| 2. |
Skulachev VP, et al. FEBS Lett 423(3):275-80, 1998. |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
