Clone ID: EP2109Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2118-1
Size: 100ul
Price: $ 285
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| A. Immunohistochemical analysis of untreated cervical carcinoma using anti-Phospho-Akt1 (pS473) RabMAb (cat. # 2118-1). |
B. Immunohistochemical analysis of Phosphatase- treated cervical carcinoma using anti-Phospho-Akt1 (pS473) RabMAb (cat. # 2118-1). |
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| C. Western blot analysis on 3T3 cell lysates using anti-Phospho-Akt1 (pS473) RabMAb (cat. #2118-1), 1:10,000 dilution. Cells were either (A) untreated or (B) treated with PDGF. |
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Recommended Applications: WB, IHC, ICC
Species Reactivity: Human, Mouse
Mol. Weight: 60 kDa
UniProt ID: P31749
Alternative names: RAC-PK-alpha, Protein kinase B, PKB, C-AKT
Description: Akt1/PKBa is a Ser/Thr protein kinase whose kinase activity plays a key role in various cellular functions, including apoptosis, glycogen synthesis, and cell growth . Akt1/PKBa is activated by various growth and survival factors (1). Phosphorylation on Thr308, Ser473 and Tyr474 is required for full activity. Akt1/PKBa inhibits apoptosis by phosphorylating and inactivating several targets, including caspase-9 (2). Akt1/PKBa regulates glycogen synthesis by phosphorylating and inactivating glycogen synthase kinase-3a and b (3,4). Three mammalian Akt1/PKBa isoforms have been identified. Specificity: A phospho specific peptide corresponding to residues surrounding Ser473 of human Akt1 was used as immunogen. The antibody detects Akt1 phosphorylated at Serine 473.
Recommended Dilutions:
WB: 1:5000 - 10,000
IHC: 1:100 250
ICC: 1:100 250
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
Burgering, B.M. and P.J. Coffer.Nature 376: 599 |
| 2. |
Cardone, M.H., et al. Science 282: 1318 |
| 3. |
Hajduch, E., et al. FEBS Lett. 492: 199 |
| 4. |
Cross, D.A., et al. Nature 378: 785-789 (1995). |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
