Clone ID: EP1045Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2064-1
Size: 100uL
Price: $ 250
Recommended Applications: WB, IHC, FC, IP
Species Reactivity: Human
Mol. Weight: 185 kDa
UniProt ID: P04626
Alternative names: p185erbB2, C-erbB-2, NEU proto-oncogene, Tyrosine kinase-type cell surface receptor HER2, MLN 19, CD340 antigen
Description: HER2 / ErbB2 is one of the four members of the ErbB receptor family of transmembrane receptor-like tyrosine kinases (1). The kinase activity of ErbB2 can be activated without ligand if it is overexpressed, and by association with other ErbB proteins (2). Overexpression of ErbB2 is detected in almost 40% of human breast cancers (3). Binding of c-Cbl ubiquitin ligase to Tyr1112 of ErbB2 leads to poly-ubiquitination of ErbB2 and enhances its degradation (4). ErbB2 is one of the major targets for the treatment of breast cancer and other carcinomas. Specificity: A synthetic peptide corresponding to residues near the C-terminus of human Her2 was used as an immunogen. This antibody detects Her2 phosphorylated at Tyr1248 as well as unphosphorlyated Her2.
Recommended Dilutions:
WB: 1:50,000 100,000
IHC: 1:100 250
Flow Cytometry: 1:30
IP: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on SKBR-3 cell lysate using anti-ErbB2/Her2 RabMAb (cat. # 2064-1), dilution 1:100,000. |
B. Immunohistochemical staining of paraffin-embedded human breast carcinoma using anti-ErbB2/Her2 RabMAb (cat. #2064-1). |
Description References:
| 1. |
Muthuswamy, S. K., et al. Mol. Cell. Biol. 19: 6845-6857 (1999). |
| 2. |
Qian, X., et al. Proc. Natl. Acad. Sci. USA 91: 1500 |
| 3. |
Dittadi, R, et al. J. Natl. Cancer Inst. 92: 1443-1444 (2000). |
| 4. |
Klapper, L. N., et al. Cancer Res. 60: 3384-3388 (2000). |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
