Clone ID: PEG-B-47
Antibody Type: Rabbit Monoclonal IgG Protein A purified
Catalog#: 2061-1
Size: 100ug
Price: $ 225
*please call for quote on bulk orders.
Recommended Applications: WB, ELISA, IHC
For more info, vist the Anti-PEG Antibodies Portal
Alternative names:
Description: Polyethylene glycol（PEG）is a family of long chain polymers attached to a glycerine backbone. It is a nonionic, nontoxic, biocompatible, strongly hydrophilic polymer, which has a large exclusion volume in aqueous solution (1). The covalent attachment of PEG is now commonly used to modify a variety of proteins and drugs (2,3). The modification of a biopharmaceutical with polyethylene glycol (PEG) increases its hydrodynamic radius, reduces immunogenicity and proteolytic cleavage. Other benefits include decelerated renal excretion, improved stability towards proteolysis and increased solubility of the biopharmaceutical in aqueous solutions (4). As examples, PEG-adenosine deaminase (Adagen) is used for the treatment of severe combined immunodeficiency syndrome, PEGasparaginase (Oncaspar) is used for the treatment acute lymphoblastic leukemia, PEGinterferon α2a (Pegasys) is used for the treatment Hepatitis C, Branched PEGanti-VEGF aptamer (Pegaptanib, Macugen) is used for the treatment Macular degeneration(age-related) (5). An Anti-PEG antibody can be used to monitor a drugs pharmacokinetics, including distribution, metabolism and excretion. In addition, it can be used for the quality control of pegylated molecules in ELISA, WB and flow cytometery. Specificity: KLH-PEG with terminal methoxy group was used as an immunogen. This antibody recognizes the terminal methoxy group of the PEG molecule.
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Immunoblot of different antigen-PEG preparations. Western Blot images using Anti-PEG 47 to detect BSA-PEG. Lane 1: 300 ng/mL; lane 2: 75 ng/mL; lane 3: 15 ng/mL; lane 4: 1.5 ng/mL. |
B. Comparison of anti-PEG-47 RabMAb and Vendor A mouse MAb in Direct ELISA assy. Goat anti-rabbit IgG-AP used for anti-PEG-47 detection; goat anti-mouse IgM-AP used for Vendor A MAb detection. Fig 1a. Direct ELISA using 1 ug/mL of PEG-GCSF. |
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| C. Comparison of sandwich ELISA using RabMAb/RabMAb (PEG 47/PEG 47) and MAb/RabMAb (Vendor A/PEG 47) for capture/ |
D. Comparison of 10 ug/well of activated linear (PEG5K) and branched (PEG40K) PEG using 5 ug/mL of anti-PEG-47 RabMAb in Direct ELISA assay. |
Description References:
| 1. |
Guiotto, A. et al. (2004) Anchimeric assistance effect on regioselective hydrolysis of branched PEGs: a mechanistic investigation. Bioorg. Med.Chem. 12, 5031 |
| 2. |
Wong, S.S. (1991) Reactive groups of proteins and their modifying agents. In Chemistry of protein conjugation and cross-linking, p. 13, CRC Press |
| 3. |
Caliceti, P. et al.(1993) Active site protection of proteolytic enzymes by poly(ethylene glycol) surface modification. J. Bioact. Comp. Polym. 8,41 |
| 4. |
Frank Leenders, celares GmbH, Berlin, Germany.(2006) PEGylation technology and biopharmaceuticals. Biopharmaceuticals. 6,39-40 |
| 5. |
Francesco M.Veronese, Gianfranco Pasut. (2005) PEGylation, successful approach |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
