Clone ID: Polyclonal
Antibody Type: Rabbit Polyclonal IgG Affinity Purified
Catalog#: 2023-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human
Mol. Weight: 55 kDa
UniProt ID: EP1932Y
Alternative names:
Description: Usual human livers contain two major ALDH isozymes, i.e., cytosolic ALDH1 and mitochondrial ALDH2(1). Human red cell aldehyde dehydrogenase (ALDH) resembles the liver cytosolic isozyme in numerous physicochemical properties. Thus, the erythrocyte enzyme, by virtue of its chemical and structural identity with the liver cytosolic enzyme, may serve as a suitable peripheral enzyme model to understand the cause and mechanism of alcohol abuse-related changes in liver cytosolic ALDH that has been found to be reduced in alcoholics (2). ALDH1A1 is known to catalyze the oxidation of retinaldehyde to retinoic acid. Overexpression of human ALDH1A1 in HEK cells led to a more than 20-fold increase in 3-deoxyglucosone dehydrogenase activity (3). Specificity: A synthetic peptide corresponding to residues within human ALDH1A1 was used as an immunogen.
Recommended Dilutions:
WB: 1:2000
IHC: 1:100 250
ICC: 1:50 100
IP: 1:40
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
 |
| A. Western blot analysis on fetal liver lysate of using anti-ALDH1A1 Rabbit Polyclonal (cat. # 2023-1), dilution 1:2000. |
B. Immunohistochemical analysis of paraffin-embedded liver using anti-ALDH1A1 Rabbit Polyclonal (cat. #2023-1). |
Description References:
| 1. |
Yoshida A, et al. Alcohol 2(1):103-6, 1985 |
| 2. |
Agarwal DP, et al. Enzyme 42(1):47-52, 1989 |
| 3. |
Collard F, et al. Biochimie 89(3):369-73, 2007 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
