Clone ID: EP1569Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1967-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, FC, IP
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 55 kDa
UniProt ID: Q13509
Alternative names: Tubulin beta-III, Tubulin beta-4
Description: Microtubules are essential parts in eukaryotic cell structures, transportation, and mitosis and consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta-tubulin binds to alpha tubulin to form tubulin heterodimer which is post-translationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). Class III beta tubulin (beta III-tubulin) is a vertebrate tubulin Isotype specific to the neurons and mammalian testis cells, making it an ideal neuronal marker. Overexpression of class III beta tubulin is associated with the resistances of microtubule-targeted cancer drugs in lung cancer cell lines, breast cancer cell lines, and ovarian tumors (3). Specificity: A synthetic peptide corresponding to the C-term of human class III β-Tubulin was used as immunogen.
Recommended Dilutions:
WB: 1:20,000
IHC: 1:50 100
ICC: 1:100 250
Flow Cytometry: 1:10
IP: 1:40
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
 |
| A. Western blot analysis on HeLa cell lysate using anti- β-Tubulin, class III RabMAb (cat. # 1967-1), dilution 1:10,000. |
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Tubulin beta class III RabMAb (cat# 1967-1) |
Description References:
| 1. |
Wang, HW et al. Nature 435: 911-915, 2005. |
| 2. |
Cleveland, DW et al. Cell 20: 95-105, 1980. |
| 3. |
Hasegawa S et al. Clin Can Res 9: 2992-2997, 2003. |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
