Clone ID: EP1238Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1935-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC
Species Reactivity: Human, Mouse
Mol. Weight: 125 kDa
UniProt ID: P46531
Alternative names: Notch 1, hN1, Translocation-associated notch protein TAN-1
Description: Notch (hN1, Motch in mouse, Xotch in Xenopus) is a family of large transmembrane receptors involved in cell to cell interactions and cell fate decisions during invertebrate and vertebrate development. A modification of Notch signaling pathway can lead to changes in cellular proliferation, differentiation, and apoptotic events (1). Notch is activated by binding to membrane-bound ligands of the Delta and Serrate/Jagged family. Activated Notch receptor is proteolytically cleaved and the Notch intracellular domain (NICD) is released. The NICD binds to CSL DNA-binding protein and the Mastermind/Lag-3 co-activator in the nucleus which activates target gene expression (2,3). Notch has been implicated in T-cell acute lymphoblastic leukemia (T-All) and Multiple Sclerosis (MS). Also, repression of Notch signaling is commonly seen in cancer (4). In mammals, four different Notch receptors has been identified (Notch 1, 2, 3, and 4). Active Notch1 has been linked to inactivation of p53-dependent transactivation by inhibiting the phosphorylation of p53 protein (5). Specificity: A synthetic peptide corresponding to the Cytoplasmic portion of human Notch1 was used as immunogen.
Recommended Dilutions:
WB: 1:500
IHC: 1:50 -100
ICC: 1:100-250
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
 |
| A. Western blot analysis using anti-NOTCH1 Rabbit Monoclonal on Hek293 cell lysate.(Cat. # 1935-1), dilution 1:500. |
B. Immunohistochemical analysis of paraffin-embedded human fetal lungs using anti-NOTCH 1 RabMAb (cat# 1935-1). |
Description References:
| 1. |
Cormier S, et al. MCB 26(9):3541-3549, 2006. |
| 2. |
Mumm JS and R Kopan, Dev. Biol. 228:151-165, 2000. |
| 3. |
Schweisguth F, Curr. Biol. 14:R129-R138, 2004. |
| 4. |
Joutel A, et al. Semin Cell Dev Biol 9(6):619-25, 1998. |
| 5. |
Kim SB, et al. Cell Death Differ. 2006 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
