Clone ID: EP1376Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1912-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human
Mol. Weight: 60 kDa
UniProt ID: P23141
Alternative names: Acyl coenzyme A:cholesterol acyltransferase, ACAT, Monocyte/macrophage serine esterase, HMSE, Serine esterase 1, Brain carboxylesterase hBr1, Triacylglycerol hydrolase, TGH, Egasyn, Retinyl ester hydrolase, REH
Description: Triacylglycerol hydrolase is a microsomal enzyme that hydrolyzes stored cytoplasmic triacylglycerol in the liver and participates in the lipolysis/re-esterification cycle during the assembly of very-low-density lipoproteins. Expression of human TGH in Escherichia coli yields a protein without enzymatic activity, which suggests that posttranslational processing is necessary for the catalytic activity (1). ACAT esterifies free cholesterol and stores cholesteryl esters in lipid droplets. Macrophage ACAT1 deficiency results in increased atherosclerotic lesion area in hyperlipidemic mice via disrupted cholesterol efflux, increased lipoprotein uptake, accumulation of intracellular vesicles, and accelerated apoptosis (2). Data show that macrophage ACAT1 influences the efflux of both cellular and lipoprotein-derived cholesterol and propose a pathway for the pro-atherogenic transformation of ACAT1(-/-) macrophages (3). Specificity: A synthetic peptide corresponding to residues on the C-terminus of human ACAT was used as an immunogen.
Recommended Dilutions:
WB: 1:10,000
IHC: 1: 250 500
ICC: 1:50 100
IP: 1:100
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
 |
| A. Western blot analysis on U937 cell lysate using anti-ACAT RabMAb (cat. # 1912-1), dilution 1:10,000. |
B. Immunohistochemical staining of paraffin-embedded human liver using anti-ACAT RabMAb (cat. #1912-1). |
Description References:
| 1. |
Alam M, et al. Biochemistry 41(21):6679-87, 2002. |
| 2. |
Dove DE, et al. Atherosclerosis 186(2):267-74, 2006. |
| 3. |
Sone DE, et al. Arterioscler Thromb Vasc Biol 25(1): 128-34, 2005. |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
