Clone ID: EP661Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1891-1
Size: 100ul
Price: $ 285
Recommended Applications: WB, IHC, IP
Species Reactivity: Human
Mol. Weight: 220 kDa
UniProt ID: Q9UM73
Alternative names: Anaplastic lymphoma kinase, CD246 antigen
Description: ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. The 2;5 chromosomal translocation occurs in most anaplastic large-cell non-Hodgkin's lymphomas arising from activated T lymphocytes. This rearrangement was shown to fuse the NPM nucleolar phosphoprotein gene on chromosome 5q35 to a previously unidentified protein tyrosine kinase gene, ALK, on chromosome 2p23 (1). In the predicted hybrid protein, the amino terminus of NPM is linked to the catalytic domain of ALK. Immunoblotting with anti-ALK antibody shows that ALK is highly expressed in the neonatal brain (2). Also expressed in the small intestine, testis, and brain but not in normal lymphoid cells, ALK shows greatest sequence similarity to the insulin receptor subfamily of kinases. Unscheduled expression of the truncated ALK may contribute to malignant transformation in these lymphomas (3). Specificity: A synthetic phosphor-peptide corresponding to residues surrounding Tyr1604 of human ALK was used as an immunogen. The antibody only detects ALK phosphorylated on Tyrosine 1604.
Recommended Dilutions:
WB: 1:5000
IHC: 1:100 - 250
IP: 1:20
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on SH-SY-5Y cell lysates using anti-Phospho-ALK (pY1604) RabMAb (cat. #1891-1), 1:5000 dilution. Cells were either (A) untreated (B) treated with PTN |
B. Immunohistochemical analysis of human Non-Hodgkins lymphoma using anti-Phospho-ALK (pY1604) RabMAb (cat. # 1891-1). |
Description References:
| 1. |
Morris SW, et al. Science 263(5151):1281-4, 1994. |
| 2. |
Iwahara T, et al. Oncogene 14(4):439-49, 1997. |
| 3. |
Benharroch D, et al. Blood 91(6): 2076-84, 1998. |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
