Clone ID: EP1098Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1870-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, FC, IP
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 38 kDa
UniProt ID: P08247
Alternative names: Major synaptic vesicle protein p38
Description: Synaptophysin is an integral membrane protein of small synaptic vesicles in brain and endocrine cells (1). Synaptophysin (syp I) is a synaptic vesicle membrane protein that constitutes approximately 7% of the total vesicle protein. Multiple lines of evidence implicate syp I in a number of nerve terminal functions (2). Synaptophysin is one of the most abundant membrane proteins of small synaptic vesicles. In mature nerve terminals it forms a complex with the vesicular membrane protein synaptobrevin, which appears to modulate synaptobrevin's interaction with the plasma membrane-associated proteins syntaxin and SNAP25 to form the SNARE complex as a prerequisite for membrane fusion. It has been shown that synaptobrevin is preferentially cleaved by tetanus toxin while bound to synaptophysin or when existing as a homodimer (3). data suggest that synaptophysin is released from the Golgi apparatus in a vesicular form, after glycosylation, and is then transported to nerve endings by a mechanism which requires integrity of microtubules (4). Specificity: A synthetic peptide corresponding to residues in human Synaptophysin was used as an immunogen.
Recommended Dilutions:
WB: 1:200,000
IHC: 1:250 500
ICC: 1:250 500
Flow Cytometry: 1:20
IP: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
 |
| A. Western blot analysis on rat brain membrane using anti-Synaptophysin RabMAb (cat. # 1870-1), dilution 1:200,000. |
B. Immunohistochemical staining of paraffin-embedded human medulloblastoma using anti-Synaptophysin RabMAb (cat. #1870-1). |
|
|
| C. Immunofluorescent staining of PC12 cells using anti-Synaptophysin RabMAb (cat. #1870-1). |
|
Description References:
| 1. |
Ozcelik T, et al. Am J Hum Genet 47(3):551-61, 1990. |
| 2. |
McMahon HT, et al. Proc Natl Acad Sci 93(10):4760-4, 1996. |
| 3. |
Reisinger C, et al. J. Neurochem 90(1):1-8, 2004. |
| 4. |
Tixier-Vidal A, et al. Neuroscience 26(3):847-61, 1988. |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
