Clone ID: EP462E
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1845-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, FC
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 35 kDa
UniProt ID: P29965
Alternative names: CD40-L, Tumor necrosis factor ligand superfamily member 5, TNF-related activation protein, TRAP, T-cell antigen Gp39, CD154 antigen
Description: TRAP cleavage (CD40 Ligand), a 35 kDa ligand, is transiently expressed on the surface of T-cells and binds to CD40, which is expressed on the surface of B-cells. This binding event leads to the differentiation, proliferation, and isotype switching of the B-cells. The physiological importance of CD40L has been demonstrated by the fact that expression of defective CD40L protein causes an immunodeficiency state characterized by high IgM and low IgG serum levels, indicating faulty T-cell dependent B-cell activation (1). Abnormalities in the CD40L gene are associated with an X-linked immunodeficiency in humans [hyper-IgM (immunoglobulin M) syndrome]. This disease is characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes (2). This CD40L is expressed on activated T cells, mostly CD4+ but also some CD8+ as well as basophils/mast cells. Cross-linking of CD40 with immobilized anti-CD40 or cells expressing CD40L induces B cells to proliferate strongly, and addition of IL-4 or IL-13 allows the generation of factor-dependent long-term normal human B cell lines and the secretion of IgE following isotype switching (3). Specificity: A synthetic peptide corresponding to residues of human CD40L was used as an immunogen.
Recommended Dilutions:
WB: 1:5000
Flow Cytometry: 1:40
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
 |
| A. Western blot analysis on MOLT-4 cell lysate using anti-TRAP RabMAb (cat. #1845-1), dilution 1:5000 |
B. Flow cytometric analysis of permeabilized MOLT-4 cells using anti-TRAP RabMAb (red) (catalog # 1845-1) or a rabbit IgG (negative) (green). |
References:
| 1. |
Singh J, et al. Protein Sci. 7(5):1124-35, 1998. |
| 2. |
Allen RC, et al. Science 259(5097):990-3, 1993. |
| 3. |
Banchereau J, et al. Annu Rev Immunol. 12:881-922, 1994 |
Orders:
877-772-2622 (US and Canada)
650-443-2276 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
