Clone ID: EP887Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1825-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC
Species Reactivity: Human, Mouse
Mol. Weight: 28 kDa
UniProt ID: Q61160
Alternative names: FAS-associated death domain protein, FAS-associating death domain-containing protein, Mediator of receptor induced toxicity
Description: Fas-associating protein with death domain (FADD) is an adaptor protein involved in the apoptosis induced by Fas and TNF-R1, and mitogen induced cell proliferation (1). FADD consists of death domain (DD) at the C-terminal and death effector domain (DED) at the N-terminal (2). DD interacts with Fas and TNF-R1, which exposes the DED portion on the FADD. Unmasked DED binds to the DED of pro-caspase-8, which activates the cysteine protease cascade (3). The development of autoimmune lymphoproliferative (lpr) diseases has been linked to inactivation of Fas-FADD interaction (4). Also, the deficiency in FADD can inhibit TRAIL-induced cell apoptosis. Specificity: A synthetic peptide corresponding to residues near the N-term of human FADD was used as immunogen.
Recommended Dilutions:
WB: 1:500
IHC: 1:100 - 250
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
 |
| A. Western blot analysis on Jurkat cell lysates using anti-FADD RabMAb (cat. # 1825-1), 1:500 dilution. |
B. Immunohistochemical analysis of paraffin-embedded human lung using anti-eIF-5alpha RabMAb (cat. # 1825-1). |
Description References:
| 1. |
Imtiyaz HZ et al, I J. Biol. Chem., Vol. 280, Issue 36, 31360-31367, 2005 |
| 2. |
Chinnaiyan AM et al. Cell 81, 505 |
| 3. |
Boldin MP et al, Cell 85, 803 |
| 4. |
Cohen PL et al. Annu. Rev. Immunol. 9, 243 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
