Clone ID: EP1017Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1791-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, IP
Species Reactivity: Human
Mol. Weight: 70 kDa
UniProt ID: P02771
Alternative names: Alpha-fetoglobulin, Alpha-1-fetoprotein
Description: Alpha-fetoprotein (AFP) is a serum glycoprotein protein produced in the liver or yolk sac of fetal staged mammals. AFP synthesis is minimal after birth and trace amount is expressed in the adult liver (1). AFP gene expression is regulated by the interactions between steroid hormone receptors and transcriptional factors in separate signal transduction pathways (2). AFP functions as a binding and transporting ligand and cell growth regulator. Elevated expression level of AFP has been implicated in colorectal, ovarian, pancreatic, testicular, and certain liver cancers. High levels of AFP is also seen in some diseases such as hepatitis and colitis. AFP is used as a screening marker for fetal abnormalities in pregnant women such as Down syndrome. Recently, AFP has been introduced as an anti-cancer drugs-ligand carrier, transporting drugs to target tumor cells, increasing anti-tumor efficiency (3). Specificity: A synthetic peptide corresponding to residues near the C-term of human AFP was used as an immunogen.
Recommended Dilutions:
WB: 1:50,000
IHC: 1:250-500
IP: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
 |
| A. Western blot analysis on HepG2 membrane using anti-AFP RabMAb (cat. # 1791-1), dilution 1:50.000 |
B. Immunohistochemical staining of paraffin-embedded human fetal liver using anti-AFP RabMAb (cat. # 1791-1). |
Description References:
| 1. |
Wang XW, et al. World J Gastroenterol 7:345-351, 2001. |
| 2. |
Chen H, et al Crit Rev Eukaryot Gene Expr, 7:11-41, 1997. |
| 3. |
Zheng AI, et al. CJC 22:108-111, 2003. |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
