Clone ID: EP454Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1775-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human
Mol. Weight: 50 kDa
UniProt ID: O96020
Alternative names:
Description: Cyclin E2 is a member of the cyclin E family that can associate with and activate Cdk2. Expression of cyclin E2 is essential for the control of the cell cycle at the late G1 and early S phase, and this protein may regulate distinct rate-limiting pathway(s) during this phase (1). Cyclin E2 specifically interacts with Cdk inhibitors of the CIP/KIP family and activates both Cdk2 and Cdk3. Expression of the viral E6 oncoprotein in normal human fibroblasts increases levels of cyclin E2, but not cyclin E1, while expression of the E7 oncoprotein upregulates both (2). Abnormally high levels of cyclin E expression have frequently been observed in human cancers (3). Specificity: A synthetic peptide corresponding to a residue of human Cyclin E2 was used as an immunogen.
Recommended Dilutions:
WB: 1:2000
IHC: 1:250 500
ICC: 1:100 250
IP: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on Jurkat celll lysate of using anti-Cyclin E2 Rabbit Polyclonal (cat. # 1775-1), dilution 1:2000 |
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Cyclin-E2 RabMAb. (cat # 1775-1) |
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| C. Immunofluorescent staining of HeLa cells using anti-Cyclin-E2 RabMAb. (cat # 1775-1) |
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Description References:
| 1. |
Lauper, N., et al. Oncogene 17: 2637 |
| 2. |
Zariwala, M., et al. Oncogene 17: 2787 |
| 3. |
Gudas, J.M., et al. Mol Cell Biol. 19: 612 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
