Clone ID: EP553Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1772-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, FC
Species Reactivity: Human, Mouse
Mol. Weight: 155 kDa
UniProt ID: Q99683
Alternative names: MAPK/ERK kinase kinase 5, MEK kinase 5, MEKK 5, Apoptosis signal-regulating kinase 1, ASK-1
Description: Apoptosis signal-regulating kinase-1 (ASK1, MEKK5, MAPKKK5) is a MAP3k type protein kinase belonging to mitogen-activated protein kinase kinase kinase (MAPKKK) family. As a regulator of JNK and p38 MAPK signaling pathways, ASK1/MEKK5 phosphorylates MMK4 to activate c-Jun N-terminal kinase (JNK) and phosphorylates MEK3 and MKK6 to activate p38. ASK1/MEKK5 is activated by oxidative stress, TNF via TRAF2, Fas via Daxx, calcium overload, and endoplasmic reticulum stress (1). ASK1/MEKK5 is inactivated by Trx, Grx, 14-3-3, and protein serine/threonine phosphatase 5 (2). While elevated level of ASK1/MEKK5 can induce apoptosis, a catalytically inactive ASK1/MEKK5 acts as an inhibitor in TNF-alpha induced apoptosis. Over-expression of ASK1/MEKK5 has also been implicated in cell differentiation, such as in keratinocyte differentiation through p38 pathway (3). ASK1/MEKK5 has been implicated as a therapeutic target for neurodegenerative disorder sand cardiac dysfunction (4) Specificity: A synthetic peptide corresponding to residues in N-terminus of human MEKK5/ASK1 was used as an immunogen.
Recommended Dilutions:
WB: 1:2000
IHC: 1:100 250
ICC: 1:100 250
Flow Cytometry: 1:40
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on HeLa cell lysate using anti-MEKK5/ASK1 RabMAb (cat. # 1772-1), dilution 1:2000 |
B. Immunohistochemical analysis of paraffin-embedded human lung carcinoma using anti-MEKK5 RabMAb (cat. # 1772-1). |
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| C. Immunofluorescent staining of HeLa cells using anti-MEKK5 RabMAb (cat. # 1772-1). |
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Description References:
| 1. |
Ichijo H, et al. Science 275:90-94, 1997 |
| 2. |
Cho S, et al. JBC 279:16050-16056, 2004. |
| 3. |
Sayama K, et al. JBC 276:999-1004, 2001. |
| 4. |
Matsukawa J, et al. J. Biochem 3:261-265, 2004. |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
