Clone ID: Polyclonal
Antibody Type: Rabbit Polyclonal IgG Affinity Purified
Catalog#: 1750-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human, Rat
Mol. Weight: 14 kDa
UniProt ID: Q5QNW6
Alternative names:
Description: Changes in chromatin structure play a key role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H2B is acetylated in the N-terminal region, primary at lysines 5, 12, 15, and 20 (3). Ubiquitylation of histone H2B at lysine 123 by Rad6 has been implicated in transcriptional activation and lysine methylation on histone H3 (4). Histone H2B phosphorylation at Ser10 (for yeast) and Ser14 (for mammalian cells) has been linked to regulation of cell apoptosis and meiosis (5). Specificity: A synthetic peptide corresponding to residues of human histone H2B was used as an immunogen.
Recommended Dilutions:
WB: 1:20,000
IHC: 1:100 250
ICC: 1:50 100
IP: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on A431 celll lysate of using anti-histone H2B rabbit Polyclonal (cat. # 1750-1), dilution 1:20,000 |
B. Immunohistochemical analysis of paraffin-embedded human kidney carcinoma using anti-histone H2B Rabbit Polyclonal (cat. #1750-1). |
Description References:
| 1. |
Braunstein M, et al. Mol. Cell. Biol. 16:4349 |
| 2. |
Workman JL, Kingston RE,. Annu. Rev. Biochem. 67: 545 |
| 3. |
Cheung, P. et al. Cell103, 263 |
| 4. |
Xiao T, et al. Mol Cell Bio 25:637-651, 2005. |
| 5. |
Ahn SH, et al. Cell Cycle 4:780-783, 2005. |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
