Clone ID: EP407Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1743-1
Size: 100ul
Price: $ 210
Recommended Applications: WB
Species Reactivity: Human
Mol. Weight: 17 kDa
UniProt ID: P01584
Alternative names: IL-1 beta, Catabolin
Description: Interleukin 1 beta (IL1 beta) is a pro-inflammatory cytokine belonging to IL1 family. There are two known forms of IL1, IL1 alpha (predominant form in mice) and IL1 beta (predominant form in humans). IL1 beta and alpha are both synthesized as precursors. The IL1 beta precursor form is not biologically active, while IL1 alpha is. Expressed mainly by stimulated macrophages and monocytes, IL1 beta precursor is cleaved by Caspase 1/ICE and other proteases (2-3). Activated IL1 alpha remains mostly in the cytosol, activated IL1 beta is widely secreted. IL1 beta is known to be a mediator in inflammatory response and to be involved in a variety of cellular activities, proliferation, differentiation and apoptosis. Recent studies have shown IL1 beta to be a major cause of severe inflammation in the mouse model of Crohns disease, implicating IL1 beta as a potential marker for this disease (4). Specificity: A recombinant human IL-1 beta protein was used as an immunogen.
Recommended Dilutions:
WB: 1:20,000 (approximately 0.05ug/lane [0.5ug/200ul] of recombinant protein was loaded on the membrane)
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis of anti-IL1 beta RabMAb (cat. # 1743-1), dilution 1:20,000. (approximately 0.05ug/lane [0.5ug/200ul] of recombinant protein was loaded on the membrane) |
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Description References:
| 1. |
March CJ, et al. Nature 315:641-647, 1985. |
| 2. |
Dinarello CA, et al. Blood 87:2095-2147, 1996. |
| 3. |
Yuan J, et al. Cell 75:641-652, 1993 |
| 4. |
Shin M, et al. Science 4:734-738, 2005. |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
