Clone ID: EP774Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1727-1
Size: 100ul
Price: $ 285
Recommended Applications: WB, IHC, ICC
Species Reactivity: Human, Mouse
Mol. Weight: 170-180 kDa
UniProt ID: P00533
Alternative names: Receptor tyrosine-protein kinase ErbB-1
Description: Epidermal growth factor receptor (EGFR) is a 170 kDa transmembrane glycoprotein receptor tyrosine kinase that, when activated by epidermal growth factor (EGF), affects cell growth and differentiation in normal and cancer cells (1). Binding of EGF or TGF alpha to EGFR activates tyrosine kinase activity of the receptor (2). The carboxy terminal tyrosine residues on EGFR, Tyr 1068, Tyr 1148, and Tyr 1173, are the major sites of autophosphorylation, which occurs as a result of EGF binding (3). EGFR overexpression is exhibited various cancer, such as glioma, colorectal carcinoma, breast carcinoma, and head and neck carcinoma (4). The phosphorylation level of EGFR is considered one of the important predicators for outcome in non-small cell lung cancer therapy (5). Specificity: A synthetic phospho-peptide corresponding to residues surrounding Tyr1092 of human EGF receptor was used as immunogen.
Recommended Dilutions:
WB: 1:500-1000
IHC: 1:250 500
ICC: 1:250 500
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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Western blot analysis on A431 cell lysate using anti-Phospho-EGFR (pY1092) RabMAb (catalog #1727-1), 1:1000 dilution. Cells were (1) untreated or (2) treated with EGF. |
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| Immunohistochemical staining of untreated (A) and Phosphatase- treated (B) paraffin-embedded breast adenocarcinoma tissue using Phospho-EGFR RabMab(catalog #1727-1). |
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| Immunofluorescent staining of untreated (C) and Phosphatase- treated (D) A431 cells using Phospho-EGFR RabMab (catalog #1727-1). |
Description References:
| 1. |
Hunter T. et al. Nature 311: 414 |
| 2. |
Reynolds FH, et al. Nature 292: 259 |
| 3. |
Helin K, et al. Oncogene 6: 825 |
| 4. |
Christensen ME, et al. Danish Med Bull 45(2):121-134, 1998. |
| 5. |
Kanematsu T, et al. Oncol Res 13(5):289-298, 2003 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
