Clone ID: Polyclonal
Antibody Type: Rabbit Polyclonal IgG Affinity Purified
Catalog#: 1722-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, FC, IP
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 95 kDa
UniProt ID: P13639
Alternative names: EF-2
Description: The eukaryotic translation Elongation Factor 2 (eEF2) is a 95 kDa member of the G-protein superfamily. Following peptide bond formation, eEF2 catalyzes translocation of the deacylated tRNA in the P-site and peptidyl tRNA in the A-site into the E- and P- sites, respectively (1). The activity of eEF2 is regulated by phosphorylation (2). To be active, eEF2 must be dephosphorylated. Phosphorylation at Thr-56 causes inactivation, resulting in the termination of mRNA translation (3). eEF2 is phosphorylated by a specific, calcium and calmodulin (Ca/CaM)-dependent eEF2 kinase (4).Specificity: Specific to synthetic peptide corresponding to residues in the C-terminus of human eEF2 protein.
Recommended Dilutions:
WB: 1:50,000
IHC: 1:100 1:25
ICC: 1:50 1:100
Flow Cytometry: 1:20
IP: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis of anti-eEF2 Rabbit Polyclonal (cat .# 1722-1), dilution 1:50,000 on HEK293. |
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-eEF2 Rabbit polyclonal antibody (cat # 1722-1). |
Description References:
| 1. |
Rapp, G.; Klaudiny, J.; Hagendorff, G.; Luck, M. R.; Scheit, K. H. : Biol. Chem. Hoppe-Seyler 370: 1071-1075, 1989. |
| 2. |
Carlberg, U., Nilsson, A. and Nygard, O. (1990) Eur. J. Biochem. 191, 639 |
| 3. |
Price, N.T., Redpath, N.T., Severinov, K.V., Campbell, D.G., Russell, J.M. and Proud, C.G. (1991) FEBS Lett. 282, 253 |
| 4. |
Ryazanov, A.G., Natapov, P.G., Shestakova, E.A., Severin, F.F. and Spirin, A.S. (1988) Biochimie 70, 619 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
