Clone ID: Polyclonal
Antibody Type: Rabbit Polyclonal IgG Affinity Purified
Catalog#: 1715-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 110-120 kDa
UniProt ID: P20936
Alternative names: GTPase-activating protein, GAP, Ras p21 protein activator, p120GAP, RasGAP
Description: RasGAP, a regulator of Ras and Rho, stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. RasGAP is a Caspase 3 substrate (1) and acts as both pro- and anti-apoptotic protein depending on the extent of its cleavage by caspases (2). At low levels of caspase activity, RasGAP is cleaved at position 455, generating an N-terminal fragment (fragment N) and a C-terminal fragment (fragment C). Fragment C alone can induce apoptosis, but this response is completely inhibited by fragment N (3). Fragment N appears to be a general blocker of apoptosis downstream of caspase activation because it inhibits caspase 9-induced cell death. At higher caspase activity, fragment N is further processed and Cleavage of fragment N abrogates its protective functions, and hence the second cleavage of RasGAP promotes apoptosis (4).Specificity: A synthetic peptide corresponding to residues in the C-terminus of human RasGAP was used as immunogen.
Recommended Dilutions:
WB: 1:1,000-5,000
IHC: 1:100 250
ICC: 1:100 250
IP: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on Rat brain lysate using anti-RasGAP Rabbit Polyclonal (cat. # 1715-1), dilution 1:5,000. |
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-RasGap Rabbit Polyclonal antibody (cat # 1715-1). |
Description References:
| 1. |
Yang, J.-Y., Michod, D., Walicki, J., and Widmann, C. (2004) Biochem. Pharmacol. 68, 1027 |
| 2. |
Yang, J.-Y., and Widmann, C. (2001) Mol. Cell. Biol. 21, 5346-5358 |
| 3. |
Yang, J.-Y., Michod, D., Walicki, J., Murphy, B. M., Kasibhatla, S., Martin, S., and Widmann, C. (2004) Mol. Cell. Biol. 24, 10425 |
| 4. |
Yang, J. Y., Walicki, J., Michod, D., Dubuis, G., and Widmann, C. (2005) Mol. Biol. Cell, 16, 3511 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
