Clone ID: EP734Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1706-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC
Species Reactivity: Human, Mouse
Mol. Weight: 130 kDa
UniProt ID: P35611
Alternative names: Erythrocyte adducin subunit alpha
Description: Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes (1-2). Adducin is a heterodimeric protein that consists of related subunits; α and β, or α and γ subunits (3). Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA) (4). Phosphorylation of adducing alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of adducing alpha (5).Specificity: A synthetic peptide corresponding to residues in the C-term of human Adducin was used as immunogen.
Recommended Dilutions:
WB: 1:5,000-50,000
IHC: 1:250-500
ICC: 1:250-500
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on Hela cell lysate using anti-K562 RabMAb (cat. # 1706-1), dilution 1:50,000. |
B. Immunohistochemical analysis of paraffin-embedded human lung using anti-Adducin (C-term) RabMAb (cat. # 1706-1). |
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| C. Immunofluorescent staining of HeLa using anti-Adducin RabMAb (C-term) (cat. # 1706-1). |
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Description References:
| 1. |
Gardner, K.; Bennett, V. : J. Biol. Chem. 261: 1339-1348, 1986. |
| 2. |
Kuhlman, P.A., C.A. Hughes, V. Bennett, and V.M. Fowler. 1996. J. Biol. Chem. 271: 7986-7991 |
| 3. |
Dong, L., C. Chapline, B. Mousseau, L. Fowler, K. Ramsay, J.L. Stevens, and S. Jaken. 1995. J. Biol. Chem. 270: 25534-25540 |
| 4. |
Palfrey, H. C., Waseem, A. (1985) J. Biol. Chem. 260, 16021-16029 |
| 5. |
Fukata et al. J. Cell Biol., Volume 145, Number 2, April 19, 1999 347-361 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
