Clone ID: EP704Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1699-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, FC, IP
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 125 kDa
UniProt ID: P53396
Alternative names: ATP-citrate (pro-S-)-lyase, Citrate cleavage enzyme
Description: ATP citrate lyase (ACL) is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of four identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. One of these products, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. NDPK has been found to phosphorylate ACL and insulin to increase phosphorylation of ACL (2).Specificity: A synthetic peptide corresponding to residues in the C-term of human ATP citrate lyase was used as immunogen.
Recommended Dilutions:
WB: 1:1,000-5,000
Flow Cytometry: 1: 30
IP: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on Hela cell lysate using anti-ATP citrate lyase RabMAb (cat. # 1699-1), dilution 1:5,000. |
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Description References:
1. Elshourbagy et al. Europ. J. Biochem. 204: 491-499, 1992.
2. Benjamin, W. B., and Singer, I. (1974) Biochim. Biophys. Acta 251, 28-42
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
