Clone ID: EP409Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1695-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, FC, IP
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 80 kDa
UniProt ID: P14778
Alternative names: IL-1R-1, IL-1RT1, IL-1R-alpha, p80, CD121a antigen
Description: The Interleukin 1 receptor type I (IL-1R1) belongs to the IL-1 receptor family (1). IL-1R1 binds to IL-1 alpha, IL-1 beta, and interleukin-1 receptor antagonist protein (IL-1RA). IL-1RA regulates IL-1 ligand biological activity by a competitive interaction for IL-1R1. Upon IL-1 stimulation, adaptor molecule MyD88 is first recruited to the IL-1R1-IL1R accessory protein complex, followed by the recruitment of two serine-threonine kinases, IRAK4 and IRAK, and the adaptor protein TRAF6, resulting in activation of the NF-kB pathway (2-3). Stimulation of IL-1R1, leads to activation of the transcription factors NF-B, ATF, and AP-1 (4-5). Specificity: A synthetic peptide corresponding to residues near the N-terminus of human IL-1R type 1 was used as immunogen.
Recommended Dilutions:
WB: 1:1,000-2,000
Flow Cytometry: 1:10
IP: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on Jurkat cell lysate using anti-IL-1R1 RabMAb (cat. # 1695-1), dilution 1:2,000. |
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Description References:
| 1. |
Sims et al. Proc. Nat. Acad. Sci. 86: 8946-8950, 1989. |
| 2. |
Li, S., Strelow, A., Fontana, E. J., and Wesche, H. (2002) Proc. Natl. Acad. Sci. U. S. A. 99, 5567-5572 |
| 3. |
Wesche, H., Henzel, W. J., Shillinglaw, W., Li, S., and Cao, Z. (1997) Immunity 7, 837-847 |
| 4. |
Barnes, P. J., and Karin, M. (1997) N. Engl. J. Med. 336, 1066-1071 |
| 5. |
O'Neill, L. A. (1997) Biochem. Soc. Trans. 25, 295-302 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
