Clone ID: EP618Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1676-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, FC, IP
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 65 kDa
UniProt ID: Q13485
Alternative names: SMAD 4, Mothers against DPP homolog 4, Deletion target in pancreatic carcinoma 4, hSMAD4
Description: Smad 4 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, eight Smads have been identified and can be divided in 3 subgroups based on their structure and functions; pathway-restricted, common mediator and inhibitory Smad. Smad 4 is the common Smad (co-Smad) (1-2). Previously identified as the tumor suppressor DPC4 (deleted in pancreatic carcinoma, locus 4), Smad 4 is functionally distinct among the Smad family, and is required for the assembly and transcriptional activation of diverse, Smad-DNA complexes (3). In contrast to the R-Smads, Smad 4 is not regulated by phosphorylation, but acts as a common mediator of TGF-β, activin, and bone morphogenetic protein signaling responses (4). Smad 4 is frequently inactivated in pancreatic, biliary and colorectal tumors (5-6). Specificity: A synthetic peptide corresponding to residues in the C-term of human Smad 4 was used as immunogen.
Recommended Dilutions:
WB: 1:1,000-5,000
IHC: 1:100-250
ICC: 1:100-250
Flow Cytometry: 1:20
IP: 1:30
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on SHSY5Y cell lysate using anti-Smad 4 (C-term) RabMAb (cat. # 1676-1), dilution 1:5,000. |
B. Immunohistochemical analysis of paraffin-embedded human lung carcinoma using anti-SMAD4 (C-term) RabMAb (cat. # 1676-1). |
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| C. Immunofluorescent staining of HeLa cells using anti-SMAD4 (C-term) RabMAb (cat. # 1676-1). |
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Description References:
| 1. |
Eppert et al. Cell 86: 543-552, 1996 |
| 2. |
Derynck et al.1996.Curr. Biol. 6: 1226-1229 |
| 3. |
Hudson er al. (1998) Development 125, 1407-1420 |
| 4. |
Lagna et al. (1996) Nature 383, 832-836 |
| 5. |
Hahn,et al. (1996) Science 271, 350-353 |
| 6. |
Goggins et al. (1998) Cancer Res. 58, 5329-5332 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
