Clone ID: EP512Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1652-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, FC, IP
Species Reactivity: Human
Mol. Weight: 18-24 kDa
UniProt ID: Q9BXH1
Alternative names: p53 up-regulated modulator of apoptosis, JFY-1
Description: PUMA, (p53 upregulated modulator of apoptosis), is activated by p53 in cells undergoing p53-induced apoptosis (1-2). Localized in the mitochondria PUMA induces cytochrome c release and activates the rapid induction of programmed cell death. PUMA interacts with Bcl2 (3), Bcl-XL, Bcl2-like-1 protein and promotes mitochondrial translocation and multimerization of BAX. PUMA also plays a role in mediating p53-induced cell death through the cytochrome c/APAF1-dependent pathway (2). Two isoform of PUMA are known; PUMA-alpha and PUMA-beta. Specificity: A synthetic peptide corresponding to residues in the C-term of human PUMA was used as immunogen. This antibody is predicted to detect both isoforms based on sequence analysis. The isoform B is ~18 kDa and isoform A is ~ 24kDa. Isoform B is primarily recognized on the western blot gel.
Recommended Dilutions:
WB: 1:1,000-5,000
IHC: 1:100-250
ICC: 1:250-500
Flow Cytometry: 1:30
IP: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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A. Western blot analysis on K562 cell lysate using anti-PUMA (C-term) RabMAb (cat. # 1652-1), dilution 1:5,000.
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B. Immunofluorescent staining of HeLa cells using anti-PUMA (C-term) RabMAb (cat. # 1652-1). |
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C. Immunohistochemical analysis of paraffin-embedded human ovary carcinoma using anti-PUMA (C-term) RabMAb (cat. # 1652-1).
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D. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-PUMA (C-term) RabMAb (cat. # 1652-1). |
Description References:
| 1. |
Yu, J., Zhang, L., Hwang, P.M., Kinzler, K.W. and Vogelstein, B., 2001. Mol. Cell 7, pp. 673 |
| 2. |
Nakano, K. and Vousden, K.H., 2001. Mol. Cell 7, pp. 683 |
| 3. |
Han, J. et al. 2001. Proc. Natl. Acad. Sci. USA 98, pp. 11318 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
