Clone ID: EP559Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1650-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human, Rat
Mol. Weight: 44 kDa
UniProt ID: P45985
Alternative names: MAP kinase kinase 4, JNK-activating kinase 1, c-Jun N-terminal kinase kinase 1, JNKK, SAPK/ERK kinase 1, SEK1
Description: MKK4, also known as JNKK1 and SEK1, is a dual specificity kinase that belongs to the Ser/Thr protein kinase family. MKK4 is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. MKK4 phosphorylate and activate p38 MAP kinase and JNK but not ERK 1/2 (1-2). JNK is activated by dual phosphorylation at Thr and Tyr by MKK4 and MKK7; although MKK4 will preferentially phosphorylate Tyr (3). MKK4 is also known to be a tumor suppressor, as mutations inactivating the MKK4 gene have been found in 5% of a wide variety of tumor types (4). Specificity: A synthetic peptide corresponding to residues near the N-term of human MKK4 was used as immunogen.
Recommended Dilutions:
WB: 1:500-2,000
IHC: 1:50-100
ICC: 1:250-500
IP: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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A. Western blot analysis on Hela cell lysate using anti-MKK4 RabMAb (cat. # 1650-1), dilution 1:2,000.
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B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-MKK4 RabMAb (cat. # 1650-1). |
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C. Immunofluorescent staining of HeLa cells using anti-MKK4 RabMAb (cat. # 1650-1).
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Description References:
| 1. |
Lin, et al. Science 268: 286-290, 1995 |
| 2. |
Sanchez I, Hughes RT, Mayer BJ, et al Nature (Lond) 1994;372:794-8 |
| 3. |
Lawler, S., Fleming, Y., Goedert, M., and Cohen, P. (1998) Curr. Biol. 8, 1387 |
| 4. |
Yoshida et al. (1999) Cancer Res. 59, 5483 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
