Clone ID: EP296Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1646-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 8.5 kDa
UniProt ID: P62988
Alternative names:
Description: Ubiquitin (originally ubiquitous imunopoeitic polypeptide-UBIP) is an 8.5 kDa protein highly conserved among eukaryotic species (1). Ubiquitin acts through its post-translational attachment (ubiquitinylation) to other proteins, where these modifications alter the function, location or trafficking of the protein and targets it for destruction by the 26S proteasome. Ubiquitinylation also termed ubiquination, is an ATP-dependent process that involves the action of at least three enzymes: E1 (activating enzyme), E2 (conjugation enzyme) and E3 (ligase) working sequentially (2-4). This process is an important regulatory tool that controls the concentration of key signaling and regulatory proteins as well as removing damaged, misfolded or mutant proteins (5). Specificity: A synthetic peptide corresponding to residues in the C-term of human Ubiquitin was used as immunogen.
Recommended Dilutions:
WB: 1:1,000
IHC: 1:250-500
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
 |
A. Western blot analysis on Hela cell lysate using anti-Ubiquitin (C-term) RabMAb (cat. # 1646-1), dilution 1:2,000.
|
B. Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using anti-Ubiquitin (C-term) RabMAb (cat. # 1646-1). |
Description References:
| 1. |
Goldstein,G., Scheid, M., Hamerling, U., Boyse, E.A., Schlesinger, D.H. & Niall, D. (1975) Proc. Natl. Acad. Sci. USA 72 1-15. |
| 2. |
Hatfield, P.M. & Vierstra, R.D. (1992) J. Biol. Chem. 267 14799-14803. |
| 3. |
Sullivan, M.L. & Vierstra, R.D. (1991) J. Biol. Chem. 266 23878-23885. |
| 4. |
Gonen, H., et al. (1996) J Biol Chem 271 302-310. |
| 5. |
Hatakeyama S. , Nakayama K.I. Biochem. Biophys. Res. Commun. 302: 635-645 (2003). |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
