Clone ID: Y531
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1622-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, FC, IP
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 47 kDa
UniProt ID: P16333
Alternative names: NCK adaptor protein 1, SH2/SH3 adaptor protein NCK-alpha
Description: Nck is a ubiquitously expressed adaptor protein consisting of one SH2 and three SH3 Src homology domains. Stimulation by epidermal growth factor (EGF) or platelet-derived growth factor (PDGF) leads to phosphorylation of Nck on tyrosine, serine and threonine residues. Overexpression of Nck resulted in transformation of NIH 3T3 and 3Y1 rat fibroblast (1-2). Additionally, Nck has been shown to associate with BLNK, PLCG1 and VAV1 in a B-cell antigen receptor-dependent fashion and may play a role in linking tyrosine and serine/threonine kinase pathways within the cell (2). Specificity: A synthetic peptide corresponding to residues close to the SH2 domain of human Nck was used as immunogen.
Recommended Dilutions:
WB: 1:500-10,000
IHC: 1:100-250
ICC: 1:250-500
Flow Cytometry: 1:60
IP: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
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| A. Western blot analysis on Hela cell lysate using anti-Nck RabMAb (cat. #1622-1) 1:10,000 dilution. |
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Nck RabMAb (cat. # 1622-1). |
Description References:
| 1. |
W Li, P Hu, EY Skolnik, A Ullrich and J Schlessinge; Mol. Cell. Biol., 12 1992, 5824-5833, Vol 12, No. 12 |
| 2. |
M M Chou, J E Fajardo, and H Hanafusa; Mol Cell Biol. 1992 December; 12(12): 5834 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
