Clone ID: Y238
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1617-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, FC, IP
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 50-55 kDa
UniProt ID: O00141
Alternative names: Serum/glucocorticoid-regulated kinase 1
Description: Serum and glucocorticoid-regulated kinase (SGK-1) is a 50kDa Serine/Threonine kinase that plays a role in the regulation of epithelial Na+ transport. Two additional SGK isoforms have been identified (SGK2 and SGK3). SGK is regulated by phosphorylation at Thr256 and Ser422 by the phosphatidyl-dependent protein kinase -1 (PDK1) and PDK-2 respectively (1-2). Nedd4-2 regulates SGK activity by catalyzing its ubiquination and degradation. SGK also regulates the binding of Nedd4-2 to the epithelial Na+ channel (ENaC) protein by phosphorylating Nedd4-2 which helps increase Na+ transport (4). While environmental stresses increases its expression, SGK is known to play a role in promoting cell survival, proliferation and differentiation (5). Specificity: A synthetic peptide corresponding to residues of human SGK was used as an immunogen.
Recommended Dilutions:
WB: 1:500
IHC: 1:50
Flow Cytometry: 1:30
IP: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
 |
A. Western blot analysis of anti-SGK RabMAb (cat. #1617-1) 1:500 dilution A- A431 B- NIH3T3
|
B. Immunohistochemical analysis of paraffin-embedded human kidney carcinoma using anti-SGK RabMAb (cat. # 1617-1). |
Product References:
| 1. | Am J Physiol Cell Physiol, Jan 2007; 10.1152/ajpcell.00399.2006., Subcellular Location of Serum- and Glucocorticoid-Induced Kinase-1 (SGK1) in Renal and Mammary Epithelial Cells ,Cordas EA et al
[Application: WB] |
Description References:
| 1. |
Kobayashi, T, Deak M, Morrice N, and Cohen P. Biochem J 344: 189-197, 1999 |
| 2. |
Liu, D, Yang X, and Songyang Z.Curr Biol 10: 1233-1236, 2000 |
| 3. |
Ruifeng Zhou and Peter M. Snyder J. Biol. Chem., Vol. 280, Issue 6, 4518-4523, February 11, 2005 |
| 4. |
Debonneville et al (2001) EMBO J. 20, 7052 |
| 5. |
Leong, M. L., Maiyar, A. C., Kim, B., O'Keeffe, B. A., and Firestone, G. L. (2003) J. Biol. Chem. 278, 5871 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
