Clone ID: Y463
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1615-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, FC, IP
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 85 kDa kDa
UniProt ID: O15111
Alternative names: I kappa-B kinase alpha, IkBKA, IKK-alpha, IKK-A, IkappaB kinase, I-kappa-B kinase 1, IKK1, Conserved helix-loop-helix ubiquitous kinase, Nuclear factor NF-kappa-B inhibitor kinase alpha, NFKBIKA
Description: IKK-alpha (I-Kappa-B kinase-alpha) is a member of the IKK complex which is composed of IKK-alpha, IKK-beta, IKK-gamma and IKAP (1-2). Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. Activation of NFKB by TNF requires phosphorylation of IKK-alpha at threonine 23 and serine 176 by AKT1 and NIK respectively (3). It has been demonstrated that IKK-alpha can accumulate in the nucleus after cytokine exposure (4). In the nucleus IKK-alpha interacts with CREB-binding protein in conjunction with RELA to be recruited to the NF-kappa-B-responsive promoters in order to mediate the cytokine-induced phosphorylation and subsequent acetylation of specific residues in histone H3 (5). Specificity: A synthetic peptide corresponding to residues in the N-term of human IKK-alpha was used as immunogen.
Recommended Dilutions:
WB: 1:10,000-50,000
IHC: 1:50
ICC: 1:500
Flow Cytometry: 1:40
IP: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
 |
A. Western blot analysis on Daudi cell lysate using anti-IKK-alpha (N-term) RabMAb (cat. #1615-1) 1:50,000 dilution
|
B. Immunohistochemical analysis of paraffin-embedded human stomach carcinoma using anti-IKK alpha (N-term) RabMAb (cat. # 1615-1). |
Description References:
| 1. |
Zandi, E.; Rothwarf, D. M.; Delhase, M.; Hayakawa, M.; Karin, M.: Cell 91: 243-252, 1997 |
| 2. |
Scheidereit, C. Docking IB kinase. Nature 395: 225-226, 1998 |
| 3. |
Ozes, O. N.; Mayo, L. D.; Gustin, J. A.; Pfeffer, S. R.; Pfeffer, L. M.; Donner, D. B. : Nature 401: 82-85, 1999.
4. Anest, V.; Hanson, J. L.; Cogswell, P. C.; Steinbrecher, K. A.; Strahl, B. D.; Baldwin, A. S. : Nature 423: 659-663, 2003 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
