Clone ID: Y391
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1612-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, FC, IP
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 289 kDa
UniProt ID: P42345
Alternative names: FK506-binding protein 12-rapamycin complex-associated protein 1, Rapamycin target protein, RAPT1, Mammalian target of rapamycin, mTOR
Description: The mammalian target of rapamycin (mTOR) is a major effector of cell growth and proliferation via the regulation of protein synthesis. mTOR is composed of an N-term; 20 tandem repeats- HEAT which are implicated in protein-protein interactions and a C-term; which includes a FAT domain, a FBR domain, a kinase domain, a NDR domain and a FATC domain. The FATC domain is essential to mTOR activity and the deletion of a single amino acid from this domain abrogates the activity. mTOR can be auto-phosphorylated via its intrinsic serine/threonine kinase activity. mTOR regulates protein synthesis through the phosphorylation and inactivation of the repressor of mRNA translation 4E-BP1 and though the phosphorylation and activation of S6 kinase. RAPTOR (regulatory associated protein of TOR) is a positive regulator of TOR. Other known mediators of mTOR include PI3-K and ATK from the insulin pathway. Specificity: A synthetic peptide corresponding to residues near the C-term of PI3K/PI4K domain of human mTOR/FRAP was used as an immunogen
Recommended Dilutions:
WB: 1:1,000-2,000
IHC: 1:250-500
ICC: 1:50-100
Flow Cytometry: 1:80
IP: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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A. Western blot analysis of anti-mTOR/FRAP RabMAb (cat. # 1612-1), dilution 1:2,000. A: Hela B: MCF-7
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B. Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using anti-mTOR/FRAP RabMAb (cat. # 1612-1). |
Description References:
| 1. |
Hay, N.; Sonenberg, N. :Genes Dev. 18: 1926-1945, 2004. |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
