Clone ID: Y486
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1600-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 22 kDa
UniProt ID: P61586
Alternative names: H12
Description: Rho belongs to the Ras super family of low molecular weight GTPases. Fifteen Rho proteins have already been characterized and divided into three sub-families; the first includes Rho (A, B, C), the second, Rnd 1-3 and the third which includes Rac 1-3, RhoG, Cdc42Hs, Rho/TTF. TC10 and Chp (1-2). Rho acts as a molecular switch which turns on of off various intracellular signaling pathways such as ACK, PAKs, MEKKs ROCK (3). Rho is active when bound to GTP and inactive when bound to GDP (4). It is also known to participate in many physiological activities including cell migration, adhesion, cytokinesis, proliferation, differentiation and apoptosis and to a greater extend cell transformation (5). Specificity: A synthetic peptide corresponding to residues in the N-term of human Rho was used as immunogen. The antibody is predicted to detect RhoA, RhoB and RhoC based on sequence analysis.
Recommended Dilutions:
WB: 1:2,000
IHC: 1:50-100
ICC: 1:100-250
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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A. Western blot analysis on PC12 cell lysate using anti-Rho RabMAb (cat. # 1600-1), dilution 1:2,000.
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B. Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using anti-Rho RabMAb (cat. # 1600-1). |
Description References:
| 1. |
Murphy et al. Nature. 384:427-432 |
| 2. |
Yeramian P, Chardin P, Madaule P, Tavitian A.Nucleic Acids Res. 1987 Feb 25;15(4):1869. |
| 3. |
Maekawa et al. Science 285: 895-898, 1999. |
| 4. |
Boguski, M.S., and F. McCormick. 1993. Nature. 366:643-654 |
| 5. |
Khosravi-Far, R., P.A. Solski, G.J. Clark, M.S. Kinch, and C.J. Der. 1995. Mol. Cell. Biol. 15:6443-6453. |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
