Clone ID: Y384
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1593-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, FC, IP
Species Reactivity: Human
Mol. Weight: 110 kDa
UniProt ID: P42338
Alternative names: PI3-kinase p110 subunit beta, PtdIns-3-kinase p110, PI3K, PI3Kbeta
Description: PI3-Kinases (PI3-Ks) are a family of lipid kinases that are implicated in signal transduction. PI3-K consists of two subunits; p85 and p10. The p85 subunit localize PI3-K activity to the plasma membrane while the p110 subunit contains the catalytic domain of PI3-K (1-2). Four isoforms of p110 has been found; α, β, γ, and the Δ subunit (3). The beta isoform forms heterodimers with p85 subunit (4). p110-beta has a role in regulating the formation and stability of integrin adhesion bonds (alpha-2B and beta-3), which are necessary for shear force-induced platelet activation (5). Specificity: A synthetic peptide of human PI3-K p110 beta was used as an immunogen.
Recommended Dilutions:
WB: 1:500
Flow Cytometry: 1:40
IP: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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A. Western blot analysis on Jurkat cell lysate using anti-PI3-kinase p110 subunit beta (N-term) RabMAb (cat. # 1593-1), dilution 1:500.
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Product References:
| 1. | J. Virol., Feb 2007; 81: 1186 - 1194., Rhinovirus Activates Interleukin-8 Expression via a Src/p110? Phosphatidylinositol 3-Kinase/Akt Pathway in Human Airway Epithelial Cells ,Bentley JK et. al.
[Application: WB] |
Description References:
| 1. |
Otsu, M., Hiles, I. D., Gout, I., Fry, M. J., Ruiz-Larrea, F., Panayatou, G., Thompson, A., Dhand, R., Hsuan, J., Totty, N., Smith, A. D., Morgan, S. J., Courtnidge, S. A., Parker, P. J., and Waterfield, M. D. (1992) Cell 65, 91-104 |
| 2. |
Hiles, I. D., Otsu, M., Volinia, S., Fry, M. J., Gout, I., Dhand, R., Panayatou, G., Ruiz-Larrea, F., Thompson, A., Totty, N. F., Hsuan, J. J., Courtnidge, S. A., Parker, P. J., and Waterfield, M. D. (1991) Cell 70, 419-429 |
| 3. |
Hu, P., Mondino, A., Skolnik, E. Y., and Schlessinger, J. (1993) Mol. Cell. Biol. 13, 7677-7688 |
| 4. |
Jackson et al. Nature Med. 11: 507-514, 2005. |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
