Clone ID: Y330
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1578-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, FC, IP
Species Reactivity: Human
Mol. Weight: 15-20 kDa
UniProt ID: Q13541
Alternative names: 4E-BP1, eIF4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, PHAS-I
Description: 4E-BP1 (eIF4E-binding protein) also known as PHAS, is a 10-12 kDa acidic protein that compete with eIF4G for binding of eiF4E to the mRNA 5 cap structure (1). Binding of the 4E-BPs to eIF4E is reversible and is dependent on the phosphorylation status of 4E-BP. Non-phosphorylated 4E-BP1 will bind strongly to eiF4E while, the phosphorylated form will not (2). Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating 4E-BP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the 4EBP1-eIF4E interaction (3-4) Specificity: A synthetic peptide corresponding to residues near the C-term of human4E-BP1 was used as immunogen.
Recommended Dilutions:
WB: 1:1,000
IHC: 1:50-100
Flow Cytometry: 1:20
IP: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on K562 cell lysate using anti-4E-BP1 RabMAb (cat. # 1578-1), dilution 1:1,000. |
B. Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using anti-4E-BP1 RabMAb (cat. # 1578-1). |
Description References:
| 1. |
Pause, A., G.J. Belsham, A.-C. Gingras, O. Donz |
| 2. |
Gingras, A.-C., S.G. Kennedy, M.A. O'Leary, N. Sonenberg, and N. Hay. 1998. Genes & Dev. 12: 502-513 |
| 3. |
Iritani, B. M., and Eisenman, R. N. (1999) Proc. Natl. Acad. Sci. U. S. A. 96, 13180 |
| 4. |
Trumpp, A., Refaeli, Y., Oskarsson, T., Gasser, S., Murphy, M., Martin, G. R., and Bishop, J. M. (2001) Nature 414, 768 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
