Clone ID: Y174
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1561-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC
Species Reactivity: Human, Mouse
Mol. Weight: 46 kDa
UniProt ID: P49841
Alternative names: GSK-3 beta
Description: Glycogen synthase kinase 3beta (GSK-3β) is a Serine/Threonine protein kinas involved in the insulin and wingless pathways(1-2). Originally identified as a regulator of glycogen synthase, it has been shown to regulate a diverse array of cellular functions. GSK-3β activity is down regulated by phosphorylation on Serine 9 by PKB, or activated by phosphorylation on Tyrosine 216. Once activated, the protein can phosphorylate p53, c-jun, heat shock factor-1 and cyclin D1 (3-4). It is also known to phosphorylate Tau in Allzheimers disease. Additionally, increased GSK-3β protein levels are found in Alzheimers disease brains. Specificity: A synthetic peptide corresponding to residues in the C-term of human GSK-3β was used as immunogen. This antibody may detect splice isoform 2 based on sequence homology.
Recommended Dilutions:
WB: 1:5,000-10,000
IHC: 1:100-250
ICC: 1:100-250
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on A431 cell lysate using anti- GSK-3β (C-term) RabMAb (cat. # 1561-1), dilution 1:10,000. |
B. Immunohistochemical analysis of paraffin-embedded human colon carcinome using anti- GSK-3β (C-term) RabMAb (cat. # 1561-1). |
Description References:
| 1. |
Plyte SE, Hughes K, Nikolakaki E, Pulverer BJ, Woodgett JR. Biochim Biophys Acta. 1992 Dec 16; 1114(2-3):147 |
| 2. |
Miller, J. R. & Moon, R. T. (1996) Genes Dev. 10, 2527-2539 |
| 3. |
Boyle WJ, Smeal T, Defize LH, Angel P, Woodgett JR, Karin M, Hunter T. Cell. 1991 Feb 8; 64(3):573 |
| 4. |
Bellacosa, A., Testa, J. R., Staal, S. P. & Tsichllis, P. N. (1992) Science 254, 244-247 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
