Clone ID: Y304
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1556-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, ICC
Species Reactivity: Human
Mol. Weight: 25 kDa
UniProt ID: P17081
Alternative names: Ras-related GTP-binding protein TC10
Description: TC10 is a member of the Rho family of GTP-binding proteins and is closely related to Cdc42 and Rac 1. Cycling between inactive and active GTP-bound state, active TC10 can directly interact with Cdc42 kinase, p21-activated protein kinases as well as other kinases (1). Constitutive expression of active TC10 mutants (TC10/Q75L) in fibroblast decrease actin stress fiber and formation of plasma membrane microspikes, while wild type TC10 has no effect on fibroblast cell morphology (2). Mostly expressed in adipose and muscle tissues, TC10 has been found to be activated by C3G (from the insulin pathway) in adipocytes (3). Specificity: A synthetic peptide corresponding to the third GTP unit of human TC10 was used as immunogen.
Recommended Dilutions:
WB: 1:1,000
ICC: 1:250-500
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
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| A. Western blot analysis on 293 cell lysate using anti-TC10 RabMAb (cat. # 1556-1), dilution 1:1,000. |
B. Immunohistochemical staining of HeLa cells using anti-TC10 RabMAb (cat. # 1556-1). |
Product References:
| 1. | Yasudaa S, Taniguchia H, Oceguera-Yanez F, et al, An essential role of Cdc42-like GTPases in mitosis of HeLa cells ,FEBS Letters 580 (2006) 3375?3380
[Application: WB, ICC] |
Description References:
| 1. |
Neudauer, C.L., Joberty, G., Tatsis, N., and Macara, I.G. (1998). Curr. Biol. 8, 1151-1160 |
| 2. |
Murphy, G.A., Solski, P.A., Jillian, S.A., Perez de la Ossa, P., D'Eustachio, P., Der, C.J., and Rush, M.G. (1999). Oncogene 18, 3831-3845 |
| 3. |
Baumann , C.A., Ribon, V., Kanzaki, M., Thurmond, D.C., Mora, S., Shigematsu, S., Bickel, P.E., Pessin, J.E., and Saltiel, A.R. (2000). Nature 407, 202-207 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
