Clone ID: Y170
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1549-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human
Mol. Weight: 370 kDa
UniProt ID: Q13315
Alternative names: Ataxia telangiectasia mutated, A-T, mutated
Description: ATM is a nuclear phosphoprotein involved in Ataxia-telangiectasia (A-T) an autosomal recessive disorder characterized by cerebellar ataxia, immune deficiencies, increased cancer predisposition, chromosomal instability and radiation sensitivity (1-2). This large protein (370 kDa) is involved in genome stability, cellular responses to DNA damage and cell cycle control. More than 100 mutations have been identified so far and are expected to inactivate the ATM protein by truncation or large deletions (3). Defects in ATM contribute to various B cell and T cell Leukemia (4). Specificity: A synthetic peptide corresponding to residues surrounding Serine 1981 of human ATM was used as immunogen.
Recommended Dilutions:
WB: 1:5,000
IHC: 1:100-250
ICC: 1:100-250
IP: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on 293 cell lysate using anti-ATM RabMAb (cat. # 1549-1) dilution 1:5,000. |
B. Immunohistochemical analysis of paraffin-embedded human carcinoma using anti-ATM RabMAb (cat. # 1549-1). |
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C. Immunofluorescent staining of 293 cells using anti-ATM RabMAb (cat. # 1549-1).
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Product References:
| 1. | Gorodetsky E, Calkins S , Ahn J, et al, ATM, the Mre11/Rad50/Nbs1 complex, and topoisomerase I are concentrated in the nucleus of Purkinje neurons in the juvenile human brain ,DNA repair 6 ( 2007 ) 1698?1707
[Application: ICC] | | 2. | Bitona S, Gropp M, Itsyksonb P, et al, ATM-mediated response to DNA double strand breaks in human neurons derived from stem cells ,DNA repair 6 (2007) 128?134
[Application: ICC] | | 3. | Sharon Biton et. al., DNA: REPLICATION, REPAIR, AND RECOMBINATION: Nuclear ATM mediates the cellular response to DNA double strand breaks in human neuron-like cells ,J. Biol. Chem., Jun 2006
[Application: ICC] |
Description References:
| 1. |
Harnden, D.G. (1994) Int. J. Radiat. Biol. 66, S13-S19 |
| 2. |
Lavin, M.F. and Shiloh, Y. (1997) Ann. Rev. Immunol. 15, 177-202 |
| 3. |
Savitsky, K., Sfez, S., Tagle, D.A., Ziv, Y., Sartiel, A., Collins, F.S., Shiloh, Y. and Rotman, G. (1995) Hum. Mol. Gen. 4, 2025-2032. |
| 4. |
Stankovic T., Weber P., Stewart G., Bedenham T., Murray J., Byrd P.J., Moss P.A.H., Taylor A.M.R.; Lancet 353:26-29(1999). |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
