Clone ID: Y164
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1542-1
Size: 100ul
Price: $ 250
Recommended Applications: WB, IHC, ICC, FC
Species Reactivity: Human
Mol. Weight: 23 kDa
UniProt ID: Q16611
Alternative names: Apoptosis regulator BAK, Bcl-2-like 7 protein
Description: Bak a pro-apoptotic analog to Bax, is a member of the Bcl-2 family of proteins. During mitochondria-regulated programmed cell death, Bak promotes apoptosis by binding Bcl-2 and inhibiting its anti-apoptotic function (1). Upon apoptotic stimulation, Bak translocates from the cytosol to the mitochondrial membrane (2). Pro-apoptotic cascade activates BID, which oligomerizes Bak into pores that result in the release of cytochrome c (2, 3). In healthy cells, Bak associate with Bcl-xL and Mcl-1 but not with Bcl-2, Bcl-w or A1 and only when freed of both Bak will induce apoptosis. Specificity: A synthetic peptide corresponding to residues near the N-term of human Bak was used as immunogen. The antibody does not cross-react with other Bcl-2 members.
Recommended Dilutions:
WB: 1:1,000-5,000
IHC: 1:250-500
ICC: 1:250-500
Flow Cytometry: 1:10
Protocols for using Epitomics Rabbit Monoclonal Antibodies
|
 |
| A. Western blot analysis on Hela cell lysate using anti-Bak RabMAb (cat. # 1542-1). dilution 1:5,000. |
B. Immunohistochemical analysis of paraffin-embedded human stomack carcinoma using anti-Bak RabMAb (cat. # 1542-1). |
Description References:
| 1. |
Greider, C.W., and Blackburn, E.H. (1985). Cell 43, 405-413. |
| 2. |
Feng J, Funk WD, Wang SS, et al. Science 1995; 269:1236-41. |
| 3. |
Greider, C.W. (1996). Telomere length regulation. Annu. Rev. Biochem. 65, 337-365. |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
