Clone ID: Y172
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1527-1
Size: 100ul
Price: $ 285
Recommended Applications: WB, IHC, ICC, IP
Species Reactivity: Human
Mol. Weight: 42 kDa
UniProt ID: P05412
Alternative names: Activator protein 1, AP1, Proto-oncogene c-jun, V-jun avian sarcoma virus 17 oncogene homolog, p39
Description: c-Jun is a component of the transcription factor AP-1 (1). The transcriptional activity of c-Jun is regulated by phosphorylation at serines 63 and 73 (1,2). Extracellular signals, including growth factors, transforming oncoproteins and UV light, stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun-dependent transcription (3,4). Specificity: A synthetic phospho-peptide corresponding to residues surrounding Ser63 of human c-Jun was used as immunogen. The antibody only detects c-Jun phosphorylated on Serine 63. Predicted to cross-react with bovine, based on sequence homology.
Recommended Dilutions:
WB: 1:5,000-10,000
IHC: 1:50-100
ICC: 1:100-200
IP: 1:50
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on NIH 3T3 cell lysate using anti-Phospho-c-Jun (pS63) RabMAb (cat. #1527-1), dilution 1:10,000. Cells were either untreated or treated with UV or Anisomycin. |
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Phospho-c-Jun (pS63) RabMAb (catalog #1527-1). |
Product References:
| 1. | PNAS, Oct 2006; 10.1073/pnas.0602557103, Jun NH2-terminal kinase (JNK) prevents nuclear -catenin accumulation and regulates axis formation in Xenopus embryos ,Liao G et. al.
[Application: Dual ImmunoBlotting (Mouse)] |
Description References:
| 1. |
Bohmann, D., et al. Human proto-oncogene c-jun encodes a DNA binding protein with structural and functional properties of transcription factor AP-1. Science 238: 1386 |
| 2. |
Smeal, T., et al. Oncogenic and transcriptional cooperation with Ha-Ras requires phosphorylation of c-Jun on serines 63 and 73. Nature 354: 494 |
| 3. |
Derijard, B., et al. JNK1: a protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain. Cell 76: 1025 |
| 4. |
Kyriakis, J.M., et al. The stress-activated protein kinase subfamily of c-Jun kinases. Nature 369: 156 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
